EVerZom offers customized services for potency assays developments and internalization assays. EverZom has developed and standardized two assays to evaluate the immunomodulatory and angiogenesis properties of exosomes.
Exosome immunomodulation assay
Figure 1: Immunomodulatory potential of EVs through the NF-κB pathway activation (left), and release of TNF-a (right).
Exosome angiogenesis assay
HUVEC are seeded in a microwell plate for 24h. Then, a scratch is made at the bottom of each well, the cells are washed, and the different conditions are initiated: the medium without growth factors and serum for negative control, and with for positive control. EVs are tested at different concentrations to establish if they activate or inhibit the growth in a dose-response manner. The cell regeneration/migration is tracked during ~72h with a picture captured every hour by an EVOS M7000.
Images and videos are analysed by a proprietary algorithm to quantify the healing speed.