EVerZom > Exosome services > Exosome characterization services and quality control > Biochemical characterization of exosomes > Omics characterization of exosomes
EVerZom provides large characterization and quantification of exosomes services with OMICS approach.
RNA sequencing
Proteomics
Proteomic detects and lists around a maximum of 2,000 proteins by EV sample, based on their peptide fragment analysis by mass spectrometry. It gives relative numbers that can serve for inter-sample comparison with a high precision, while intra-sample comparison between different proteins is more approximative. While the gold standard is biological replicate (N=3), it is also possible to replace it by technical replicate (n=3), with a lower confidence. Single measure would be enough to obtain a list of present proteins, but quantification is thus extremely approximative and statistical comparison impossible.
Depending on the scientific question, proteomics can provide important data: from the raw list of detected proteins, it is useful for the comparison between processes, producing cells, or batches providing specific signatures. Classification by protein family provides an overview of EV content. EV-associated protein detection confirms the nature of purified exosomes while impurities such as apolipoproteins, serum protein aggregates, cell compartments can be detected.
Due to their very low abundance, proteomics is not the method of choice for cytokines and growth factor quantification, compared to Luminex analysis.
Lipidomics
EV samples are optimally constituted from 5×1010 EV, in less than 200µL (meaning [C]=2.5×1011 EV/mL of ammonium bicarbonate 150 mM buffer). But it is possible to get some results from up to 5-10 time less EV content.
Thanks to lipids quantification, it is possible to know the percentage of lipids coming for instance from lipoproteins (mainly constituted from cholesterol ester, absent from cell membrane).
We use shotgun lipidomics (without preliminar chromatography), except if a better sensitivity against precise lipid entities is required.
EVerZom provides also exosome characterization services to measure specific biomarkers like tetraspanins and exosome macromolecules dosages.