EV, exosomes and microvesicles, have been shown to contain potential RNA
such as miRNA or mRNA that may be used in the diagnosis of various biomarkers. They
are also a vehicle of choice for charging therapeutic nucleic acid (siRNA,
plasmid DNA…). Quantitative Polymerase-Chain-Reaction (qPCR) is the best method
to quantify a specific DNA or RNA (if coupled to a reverse transcription)
sequence from an EV sample after extraction. qRT-PCR allows confirming or not
the presence of a RNA/DNA of interest in EV preparation, or comparing the
efficiency of loading in EV between preparations.