--- title: "mTHPC-Loaded Extracellular Vesicles Significantly Improve mTHPC Diffusion and Photodynamic Activity in Preclinical Models" url: "https://everzom.com/2022/11/04/mthpc-loaded-extracellular-vesicles-significantly-improve-mthpc-diffusion-and-photodynamic-activity-in-preclinical-models/" description: "Pharmaceutics; 2020 Marie Millard, Solène Posty, Max Piffoux, Jordane Jasniewski, Henri-Pierre Lassalle, Ilya Yakavets, Florence Gazeau, Claire Wilhelm…" updated: "2022-11-04" --- - 4 November 2022 - < 1 mins. read # mTHPC-Loaded Extracellular Vesicles Significantly Improve mTHPC Diffusion and Photodynamic Activity in Preclinical Models - [Our publications](https://everzom.com/category/publications/) - **Journal and date** : Pharmaceutics; 2020 - **Authors** : Marie Millard, Solène Posty, Max Piffoux, Jordane Jasniewski, Henri-Pierre Lassalle, Ilya Yakavets, Florence Gazeau, Claire Wilhelm, Amanda K. A. Silva, Lina Bezdetnaya - **Abstract** : Extracellular vesicles (EVs), derived from the cell, display a phospholipid bilayer membrane that protects the cargo molecules from degradation and contributes to increasing their stability in the bloodstream and tumor targeting. EVs are interesting in regard to the delivery of photosensitizers (PSs) used in the photodynamic therapy (PDT), as they allow us to overcome the limitations observed with liposomes. In fact, liposomal formulation of meta-tetra(hydroxyphenyl)chlorin (mTHPC) (Foslip®), one of the most potent clinically approved PSs, is rapidly destroyed in circulation, thus decreasing in vivo PDT efficacy. mTHPC-EV uptake was evaluated in vitro in a 3D human colon HT-29 microtumor and in vivo study was performed in HT-29 xenografted mice. The obtained data were compared with Foslip®. After intravenous injection of the mTHPC formulations, biodistribution, pharmacokinetics and PDT-induced tumor regrowth were evaluated. In a 3D model of cells, mTHPC-EV uptake featured a deeper penetration after 24h incubation compared to liposomal mTHPC. In vivo results showed a considerable improvement of 33% tumor cure with PDT treatment applied 24h after injection, while 0% was observed after Foslip®/PDT. Moreover, 47 days were required to obtain ten times the initial tumor volume after mTHPC-EVs/PDT compared to 30 days for liposomal mTHPC. In conclusion, compared to Foslip®, mTHPC-EVs improved mTHPC biodistribution and PDT efficacy in vivo. We deduced that a major determinant factor for the improved in vivo PDT efficacy is the deep mTHPC intratumor penetration. **Link to article :** Share this article on [Maxence Duchemin](https://everzom.com/author/mduchemin/) Dr. Maxence Duchemin a research project manager at EVerZom, in charge of our projects with our partners. He is a biologist with a PhD in immunology from Inserm where he worked on developing an immunotherapy again HIV and has a working experience as a support scientist from Euroimmun. Related articles - [Our publications](https://everzom.com/category/publications/) - < 1 mins. read ## [Local administration of stem cell-derived extracellular vesicles in a thermoresponsive hydrogel promotes a pro-healing effect in a rat model of colo-cutaneous post-surgical fistula](https://everzom.com/2022/11/04/local-administration-of-stem-cell-derived-extracellular-vesicles-in-a-thermoresponsive-hydrogel-promotes-a-pro-healing-effect-in-a-rat-model-of-colo-cutaneous-post-surgical-fistula/) Journal and date : Nanoscale; 2021 Authors : Arthur Berger, Irami Araújo-Filho, Max Piffoux, Alba Nicolás-Boluda, Alice... - [Maxence Duchemin](https://everzom.com/author/mduchemin/) - 4 November 2022 - [Our publications](https://everzom.com/category/publications/) - 2 mins. read ## [Development of extracellular vesicle-based medicinal products: Aposition paper of the group ‘‘Extracellular Vesicle translatiOn to clinicaLperspectiVEs – EVOLVE France”](https://everzom.com/2022/11/04/development-of-extracellular-vesicle-based-medicinal-products-aposition-paper-of-the-group-extracellular-vesicle-translation-to-clinicalperspectives-evolve-france/) Journal and date : Advanced Drug Delivery Reviews; 2021 Authors : Amanda K.A. Silva, Marie Morille, Max Piffoux,... - [Maxence Duchemin](https://everzom.com/author/mduchemin/) - 4 November 2022 - [Our publications](https://everzom.com/category/publications/) - < 1 mins. read ## [Engineering and loading therapeutic extracellular vesicles for clinicaltranslation: A data reporting frame for comparability](https://everzom.com/2022/11/04/engineering-and-loading-therapeutic-extracellular-vesicles-for-clinicaltranslation-a-data-reporting-frame-for-comparability/) Journal and date : Advanced Drug Delivery Reviews; 2021 Authors : Max Piffoux, Jeanne Volatron, Kondareddy Cherukula,... - [Maxence Duchemin](https://everzom.com/author/mduchemin/) - 4 November 2022